Scientific Alert Note

Sequencing the cod genome for quality traits - a success story

One of the objectives of the BIOQUAL project is to establish genetic tools in order to identify quality traits in finfish aquaculture and further to produce gene microarrays to aid in broodstock selection based on quality traits. As part of these studies genetic variation among wild cod from more than 15 cod stocks spanning the worldwide distribution of the species was investigated. A surprisingly high number of genes with divergent patterns of variation among populations was found. The candidate genes identified can form the foundation for selection programmes targeting specific traits of interest to ensure improved future quality of farmed cod.

 

Author: Eěnar Eg Nielsen, DTU Aqua - National Institute of Aquatic Resources, Denmark

 


 

Cod farming scheme (click picture to enlarge)

The Atlantic cod is by many considered ‘the new salmon’ in aquaculture. A lot of effort in many countries is currently aimed at producing market size cod of good quality for human consumption. To improve production traits and quality, breeding programmes have been established. However, relatively little emphasis is given to the origin of fish for the initial establishment of ‘broodstocks’. This is despite the fact that the Atlantic cod is known to be separated into a number of genetically isolated wild stocks, which displays trait differences shaped by their genetic adaptation to the local environment (e.g. temperature and salinity). Accordingly, different stocks can potentially possess different traits beneficial for aquaculture.

 

Investigating trait differences among fish from numerous stocks simultaneously under controlled environmental conditions is, however, a formidable task. Alternatively, a ‘genome scan’ is a newly developed and fast method for generating insights into genetically based adaptive trait variation among stocks. Here a high number of genes are screened to identify those displaying divergent patterns of variation in different stocks, thus likely reflecting differences in adaptive traits.

 

Within the project BIOQUAL we investigated genetic variation among wild cod from more than 15 cod stocks spanning the worldwide distribution of the species. We studied variation in more than 100 genes of which many were candidate genes for ‘quality’, both in a narrow and a broad sense, i.e. in genes directly linked to flesh quality but also genes important for the well being and productivity of the fish.

 

We found a surprisingly high number of genes with divergent patterns of variation among populations, likely to reflect adaptive trait variation. Accordingly, broodstock selection from a single stock is highly unlikely to be representative of the trait variation desired in aquaculture. Furthermore, different broodstocks particularly suited for specific environmental conditions could be selected. Finally, the candidate genes identified here can form the foundation for selection programmes targeting specific traits of interest to ensure improved future quality of farmed cod.

 

Scanning the cod genome for evidence of selection (click picture to enlarge)